Principle of Detection According to the principle of fluorescent PCR technology, specific primers and Taqman probes are designed for the target genes ORF1ab and N genes, and detected by a fluorescent PCR detector to achieve qualitative detection . The kit uses the mRNA of ribonuclease P (RNase P, RNP), which widely present in normal human epithelial cells as a normal human cell control to monitor the extraction and detection process.